De52 whatman manual

An inexpensive resin is Whatman DE52 DEAE-cellulose. Be careful when handling it not to mix it around too vigorously because it will break up into small particles (fines) that will slow down the. supernatant was collected by centrifugation. The solution was loaded on an anion exchange column (DE52, Whatman) and the column was washed with 50 mM potassium phosphate buffer (pH ) containing 1mM camphor and 50 mM KCl. A recombinant form of Pcam was eluted with 50 mM potassium phosphate buffer (pH ) containing 1mM camphor and mM KCl. The supernatant was adjusted to pH with 1 m Tris (free base form), diluted fold with water, and applied to a DE52 anion-exchange column ( × 10 cm, Whatman) that had been equilibrated with 10 m m G3P.

supernatant was collected by centrifugation. The solution was loaded on an anion exchange column (DE52, Whatman) and the column was washed with 50 mM potassium phosphate buffer (pH ) containing 1mM camphor and 50 mM KCl. A recombinant form of Pcam was eluted with 50 mM potassium phosphate buffer (pH ) containing 1mM camphor and mM KCl. The column was packed by pumping a slurry of DE52 (Whatman) into the PREP column at 15 p.s.i. and operated at ∼10 p.s.i., to give a flow-rate of ∼30 cm/h (1 p.s.i.= Pa). Chromatography of an analytical loading of egg-white proteins is shown in Fig. 4b. Phage Purification Using Whatman ® DE52 SM buffer: 50mM Tris-HCl (pH), mM NaCl, 8mM MgSO 4 Pick a single plaque into 1ml SM buffer and incubate overnight at 4°C.

Bdcillus"S) and "Bergey's Manual of Determinative Bac- teriology. First Chromatography of the CGTase with a Column of DEAE-cellulose (Whatman DE). Anion-exchange chromatography on Whatman DE52 cellulose (50 by cm); colicin manual of quantitative immunoelectrophoresis. Uni-. Manual of Determinative Bacteriology (Wil- liams Wilkins, Baltimore, Md., (Whatman DE) column equilibrated ume of water, a white insoluble material.